LOCUS       40924_17542        3423 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pENTRHBM-CBDBMP4-TVMV DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Imai S, Li Z, Miyagawa Y, Toyoda M, Umezawa A, Mon H, Kusakabe T, 
            Yoshimura K, Lee JM.
  TITLE     Biologically active human bone morphogenetic protein 4-fused to 
            collagen binding domain produced in silkworm-baculovirus expression 
            system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Lee JM, Li Z, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2013) Contact:Jae Man Lee Laboratory of Silkworm 
            Science, Kyushu University Graduate School of Bioresource and 
            Bioenvironmental Sciences; 6-10-1 Hakozaki, Higashi-ku, Fukuoka 
            812-8581, Japan E-mail :jaemanle@agr.kyushu-u.ac.jp Phone 
            :81-92-642-2842 Fax :81-92-642-2842
REFERENCE   3  (bases 1 to 3423)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2013) Contact:Jae Man Lee Laboratory of Silkworm Science, 
            Kyushu University Graduate School of Bioresource and 
            Bioenvironmental Sciences"; volume: " 6-10-1 Hakozaki, Higashi-ku, 
            Fukuoka 812-8581, Japan E-mail :jaemanle@agr.kyushu-u.ac.jp Phone 
            :81-92-642-2842 Fax "; pages: "81-92-642-284"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     sig_peptide     499..561
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             1246..1260
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     protein_bind    complement(1652..1751)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1874..2680
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2773..3361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttcg aaggagatag aaccaattct
      481 ctaaggaaat acttaaccat gaaattctta gtcaacgttg cccttgtttt tatggtcgta
      541 tacatttctt acatctatgc ggatccagca gctgtttacc aaccgcagcc tcacccccag
      601 cctcctccct atggccactg tgtcacagac agtggtgtgg tctactctgt ggggatgcag
      661 tggctgaaga cacaaggaaa taagcaaatg ctttgcacgt gcctgggcaa cggagtcagc
      721 tgccaagaga cagctgtaac ccagacttac ggtggcaact caaatggaga gccatgtgtc
      781 ttaccattca cctacaatgg caggacgttc tactcctgca ccacagaagg gcgacaggac
      841 ggacatcttt ggtgcagcac aacttcgaat tatgagcagg accagaaata ctctttctgc
      901 acagaccaca ctgttttggt tcagactcga ggaggaaatt ccaatggtgc cttgtgccac
      961 ttccccttcc tatacaacaa ccacaattac actgattgca cttctgaggg cagaagagac
     1021 aacatgaagt ggtgtgggac cacacagaac tatgatgccg accagaagtt tgggttctgc
     1081 cccatggctg cccacgagga aatctgcaca accaatgaag gggtcatgta ccgcattgga
     1141 gatcagtggg ataagcagca tgacatgggt cacatgatga ggtgcacgtg tgttgggaat
     1201 ggtcgtgggg aatggacatg cattgcctac tcgcagcttc gagtcgacga cgatgataag
     1261 cgtagcccta agcatcactc acagcgggcc aggaagaaga ataagaactg ccggcgccac
     1321 tcgctctatg tggacttcag cgatgtgggc tggaatgact ggattgtggc cccaccaggc
     1381 taccaggcct tctactgcca tggggactgc ccctttccac tggctgacca cctcaactca
     1441 accaaccatg ccattgtgca gaccctggtc aattctgtca attccagtat ccccaaagcc
     1501 tgttgtgtgc ccactgaact gagtgccatc tccatgctgt acctggatga gtatgataag
     1561 gtggtactga aaaattatca ggagatggta gtagagggat gtgggtgccg ctcgagtgca
     1621 ggcgaaaccg tgcgcttcca gggaaatcta gacccagctt tcttgtacaa agttggcatt
     1681 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1741 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1801 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1861 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1921 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1981 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2041 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2101 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2161 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2221 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2281 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2341 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2401 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2461 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2521 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2581 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2641 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2701 gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat
     2761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3421 gtt
//
